CATALASE AND THE DECOMPOSITION OF H2O2 Essay

NAME :
CLASS : DBT 4A
DATE SUBMITTED : 14 AUG 2015

EXPERIMENT 4: CATALASE AND THE DECOMPOSITION OF H2O2 (VIRTUAL)

PROCEDURE

1. Fill the beaker with water and 5ml of hydrogen peroxide.
2. Pipette 1ml of catalase enzyme into the beaker.
3. After 10 seconds, add sulfuric acid into the beaker.
4. Using titration method, check the presence of hydrogen peroxide in beaker.
5. Fill the burette with 5ml of potassium permanganate ( KMnO4).
6. Add KMnO4 drop by drop into the beaker until the H2O2 become purple color.
7. Record the data.
8. Repeat the step 1 until step 7 for 30, 60, 90, 120 and 180 seconds.

RESULTS
Determination of catalase activity

Time (s) 10 30 60 90 120 180
Initial reading of KMnO4 (ml)

5
5
5
5
5
5
Final reading of KMnO4 (ml)
0.5
1.5
3
4
4.5
4.9
Amount of H2O2 that reacts with KMnO4 (ml)
4.5
3.5
2
1
0.5
0.1
Amount of H2O2 that was broken down by catalase
0.5

1.5
3
4
4.5
4.9
Amount of KMnO4 (ml) used
4.5
3.5
2
1
0.5
0.1
Rate determination
Time intervals (s) 0 – 10 10 – 30 30 – 60 60 – 90 90 -120 120 – 180
Rate (ml/s) 0.45
0.18 0.07 0.03 0.02 1.67 X 10-3

Calculation :
Amount of H2O2 that reacts with KMnO4 (ml) = Rate (ml/s)
Time

1) 4.5ml
…….
Word Count:
517
Page Count:
6

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